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Clc chloride channel
Identifiers
Symbol	Voltage_CLC
Pfam	PF00654
InterPro	IPR014743
SCOP	1kpl
TCDB	1.A.11
OPM family	10
OPM protein	1ots
Available PDB structures: 1kplC:88-438 2fedA:88-438 2fecB:88-438 1kpkF:88-438 2feeA:88-438 2exyB:88-438 1otuB:88-438 1otsA:88-438 1ottB:88-438 2ez0B:88-438 2exwB:88-438

Chloride channels are a superfamily of poorly understood ion channels consisting of approximately 13 members.

Chloride channels display a variety of important physiological and cellular roles that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Based on sequence homology the chloride channels can be subdivided into a number of groups. The importance of one such group, the CLC family of chloride channels, can be seen from the diseases that develop when the channel does not function normally.

This family of ion channels contains 10 or 12 transmembrane helices. Each protein forms a single pore. It has been shown that some members of this family form homodimers. In terms of primary structure, they are unrelated to known cation channels or other types of anion channels. Three ClC subfamilies are found in animals. ClC-1 (UniProt P35523) is involved in setting and restoring the resting membrane potential of skeletal muscle, while other channels play important parts in solute concentration mechanisms in the kidney [3]. These proteins contain two Pfam PF00571 domains.

Contents 1 Pathology 2 Functions 3 Commercial Applications 4 Human genes 5 See also 6 External links 7 References 8 Further reading

Bartter's syndrome, which is associated with renal salt wasting and hypokalemic alkalosis, is due to the defective transport of chloride ions and associated ions in the thick ascending loop of Henle. CLC-Kb has been implicated.

Another inherited disease that affects the kidney organs is Dent's Disease, characterised by low molecular weight proteinuria and hypercalciuria where mutations in CLC-5 are implicated.

Thomsen disease is associated with domininate mutations and Becker disease with recessive mutations in CLCN1.

Chloride channels are important for setting cell resting membrane potential and maintaining proper cell volume. These channels conduct Cl^- as well as other anions such as HCO₃^-, I^-, SCN^-, and NO₃^-. The structure of these channels are not like other known channels. Chloride channel subunits contain between 1 and 12 transmembrane segments. Some members of this family are activated by voltage, while others are activated by Ca²⁺, extracellular ligands, and pH among other modulators.^[1]

Chloride channels are disrupted in fleas, causing death, with some organic materials. Selamectin is the active ingredient in Revolution, a topical insecticide and antihelminthic used on dogs and cats. Selamectin works by replacing glutamate which normally interacts with receptors that open chloride channels at muscle synapses found in parasites. Unlike glutamate, selamectin activates the chloride current without desensitization, thereby producing prolonged hyperpolarization and impaired muscle contraction.

• CLCN1, CLCN2, CLCN3, CLCN4, CLCN5, CLCN6, CLCN7
• CLCNKA, CLCNKB
• CLIC1, CLIC2, CLIC3, CLIC4, CLIC5, CLIC6
• CLNS1A, CLNS1B
• CLCA1, CLCA2, CLCA3, CLCA4

• Cystic fibrosis transmembrane conductance regulator

• UMich Orientation of Proteins in Membranes families/superfamily-10 - Clc Chloride channels
• MeSH Chloride+channels

• 1. ^ Suzuki M., Morita T. and Iwamoto, T. (2006) Diversity of Cl(-) channels. Cell Mol Life Sci. 63(1):12-24. Entrez PubMed 16314923

• [1]. Reconstitution of functional voltage-gated chloride channels from complementary fragments of CLC-1. Schmidt-Rose T, Jentsch TJ; J Biol Chem 1997;272:20515-20521. PubMed
• [2]. Mutations in the human skeletal muscle chloride channel gene (CLCN1) associated with dominant and recessive myotonia congenita. Zhang J, George AL Jr, Griggs RC, Fouad GT, Roberts J, Kwiecinski H, Connolly AM, Ptacek LJ; Neurology 1996;47:993-998. PubMed
• [3]. ClC chloride channels. Mindell JA, Maduke M; Genome Biol 2001;2:REVIEWS3003. PubMed

v • d • e Membrane transport protein: ion channels
Ca2+: Calcium channel	Voltage-dependent calcium channel (L-type/Cavα(1.1, 1.2, 1.3, 1.4), N-type, P-type/Cavα(2.1), Q-type, R-type, T-type, β-subunits (β1, β2, β4), γ-subunits (γ2) • Inositol triphosphate receptor • Ryanodine receptor • Cation channels of sperm • Two-pore channel
Na+: Sodium channel	Navα (1.1, 1.2, 1.4, 1.5, 1.7, 1.9) • Navβ (1, 3, 4) • Epithelial sodium channel
K+: Potassium channel	Voltage-gated (Kv(1.1, 1.2, 1.3, 1.4, 1.5, 2.1, 4.2, 4.3, 7.1, 7.2, 7.3, 7.4, 10.1, 11.1/hERG), Shaker gene, KCNE1 • Calcium-activated (BK channel, SK channel, SK3) • Inward-rectifier Kir (1.1, 2.1, 2.2, 2.3, 3.1, 3.2, 3.4, 4.1, 4.2, 6.1, 6.2)) • Tandem pore domain K2P (1, 2, 3, 4, 6, 9)
Cl-: Chloride channel	Cystic fibrosis transmembrane conductance regulator
Porin	Aquaporin (1, 2, 3, 4) • Voltage-dependent anion channel
Cations: TRP	TRPA (1) • TRPC (1, 2, 3, 4, 4AP, 5, 6, 7) • TRPM (1, 2, 3, 4, 5, 6, 7, 8) • TRPML (Mucolipin-1) • TRPP (1, 2)• TRPV (1, 2, 3, 4, 5, 6)
Other/general	Voltage-gated ion channel • Ligand-gated ion channel • Cyclic nucleotide-gated ion channel (α1, α3, β3, H1, H2, H4) • Stretch-activated ion channel